Southampton trio James Ward-Prowse, Nathan Redmond and Jack Stephens all featured for England Under-21s at St Mary's on Wednesday night.
Stephens netted the winning goal in stoppage time, as the Young Lions came from 2-1 down to seal an impressive win against the Italians.
Ward-Prowse captained Aidy Boothroyd's side on the south coast, as the Young Lions began their preparations for next summer's Under-21 European Championships.
They got off to the perfect start too, with Redmond playing an important role in helping Demarai Gray open the scoring in just the sixth minute.
But the Italians responded well, and drew level in fortuitous circumstances, as Andrea Conti's cross deflected off Brendan Galloway and looped in over Angus Gunn.
The visitors then took the lead, as Federico Di Francesco found space in the six-yard box from a corner, and he tapped the ball into the empty net.
After Gray and substitute Will Hughes had both been denied by the woodwork, the Young Lions drew level, as Lewis Baker finished well from the edge of the box.
Hughes had the chance to win it for the hosts late on, but goalkeeper Pierluigi Gollini denied him from close range.
It looked as though it would end level, but Stephens popped up in stoppage time to net from Ward-Prowse's corner and seal the win.
The trio will now head off to France for another friendly, as they continue to prepare for next summer's tournament.